GSM2228383: WT rep2; Mus musculus; RNA-Seq - SRA

SRX1902811: GSM2228383: WT rep2; Mus musculus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 22.8M spots, 4.6G bases, 2.8Gb downloads

Submitted by: NCBI (GEO)

Study: Med1 is Necessary for Cardiac Function

PRJNA328164 • SRP078070 • All experiments • All runs

show Abstracthide Abstract

Analysis of ventricular derived mRNA from Med1fl/fl and Med1fl/fl cardiac knockout mice. Results provide insight into the molecual rmechanisms underlying dilated cardiomyopathy. Overall design: Methods: Ventricular samples (4 per group) from 21-day-old Med1fl/fl and Med1 cardiac knockout mice were used to generate polyA enriched stranded RNA libraries followed by RNAseq using the Illumina HiSeq platform. Raw sequence reads were analyzed with BaseSpace (www.illumina.com) by aligning reads to the mus musculus mm10 genome using the TopHat Alignment app. Transcripts were assembled and significant differentially expressed genes were determined with the Cufflinks Assembly and DE app using a false discovery rate <0.05. qRT–PCR validation was performed using SYBR Green assays

Sample: WT rep2

SAMN05364454 • SRS1545034 • All experiments • All runs

Organism: Mus musculus

Library:

Instrument: Illumina HiSeq 2000

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: PAIRED

Construction protocol: Ventricles were removed, flash frozen in liquid nitrogen, and total RNA was harvested using Trizol reagent. polyA RNA was enriched and TruSeq RNA stranded libraries were prepared for sequencing using standard Illumina protocols

Experiment attributes:

GEO Accession: GSM2228383

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 22.8M spots, 4.6G bases, 2.8Gb

Run	# of Spots	# of Bases	Size	Published
SRR3749042	22,796,040	4.6G	2.8Gb	2017-08-25

ID:: 2751049

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